Expression of alkaline phosphatase by skeletal muscle fibers of the mdx mouse. Cross-sections of diaphragm from control (A-D) and mdx (E-K) 16 weeks old mice were stained with anti-laminin for basal lamina labeling and detected with rhodamine-conjugated secondary antibodies (B, F), and with ELF-97 detection kit for ALP activity (C, G). Merge of ALP (blue) and basal lamina staining (red) from the same fields is shown (D, H). In panel J, the merge from cross-sections of diaphragm from mdx 16 weeks old mice stained with anti-embryonic myosin detected with fluorescein-conjugated secondary antibodies (green) and with anti-laminin detected with rhodamine-conjugated secondary antibodies (red) is shown. Merge of ALP staining (blue) and basal lamina staining (red) from the same fields is shown (K). Phase contrast is shown at the left (A, E, I). Bar = 25 μm.