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Table 1 Effect of Chlorate on [35S]-SO4 Incorporation into Proteoglycans and Glycosaminoglycans in C2C12 Myoblasts.

From: Inhibition of extracellular matrix assembly induces the expression of osteogenic markers in skeletal muscle cells by a BMP-2 independent mechanism

  cpm/mg DNA
  Control Chlorate
Medium   
Proteoglycans 3950 ± 520 240 ± 40
Glycosaminoglycans 1990 ± 570 520 ± 110
Extracts   
Proteoglycans 11180 ± 550 820 ± 20
Glycosaminoglycans 3820 ± 460 290 ± 100
  1. C2C12 cells were incubated for 48 hours with 30 mM sodium chlorate. Sulfate incorporation during the last 18 hours was measured in medium and detergent extracts. [35S]Sulfate radioactivity for total (proteoglycans plus glycosaminoglycans) and trichloroacetic acid-precipitated material (proteoglycans) samples, spotted on filters containing the cationic detergent cetyl pyridinium chloride ( CPC), was measured with liquid scintillation counting. DNA content was measured in same aliquots of detergent extracts (13.3 μg/μl for control and 18.0 μg/μl for chlorate treated cells). The specific activity values correspond to the means ± SD obtained from three different plates.