Figure 4
Sbh1p co-immunoprecipitates with S. cerevisiae reticulon-homologues Rtn1p, Rtn2p and Yop1p. A, The sbh1Δ RTN1-3XHA RTN2-9xmyc cells (H3723) or in B, sbh1Δ RTN1-3XHA YOP1-9xmyc cells (H3722) were transformed with YEpBIO-SBH1, YEpBIO-SBH1-TM, or an empty plasmid p426ADH. Lysates were prepared and subjected to pull-down with streptavidin-conjugated magnetic beads followed by SDS-PAGE and Western blotting. Proteins were detected with HRP-conjugated streptavidin and antibodies to Sec61p, myc and HA.