Table 2 Yeast expression vectors used in this study
Plasmid name | Insert | Marker | Source |
|---|---|---|---|
pVT102U | - | URA3 | [32] |
YEpSBH1 | SBH1(1-82) | URA3 | This study |
YEpSBH1-TM | SBH1(50-75) | URA3 | This study |
YEpBIO-(FL)SBH1 | BIO-SBH1(1-82) | URA3 | This study |
YEpBIO-SBH1-TM | BIO-SBH1(50-75) | URA3 | This study |
YEpBIO-SBH1FL(DM) | BIO-SBH1(1-82, P54S V57G) | URA3 | This study |
YEpBIO-SBH1TM (DM) | BIO-SBH1(50-75, P54S V57G) | URA3 | This study |
YEpBIO-SBH1TM(SM1) | BIO-SBH1(50-75, P54S) | URA3 | This study |
YEpBIO-SBH1TM(SM2) | BIO-SBH1(50-75, V57G) | URA3 | This study |
YEpSBH1-FL(DM)L | BIO-SBH1(1-82, P54S V57G) | LEU2 | This study |
YEpSBH1-TM(DM)L | BIO-SBH1(50-75, P54S V57G) | LEU2 | This study |
YEpSBH1-TM(SM1)L | BIO-SBH1(50-75, P54S) | LEU2 | This study |
YEpSBH1-TM(SM2)L | BIO-SBH1(50-75, V57G) | LEU2 | This study |
p425ADH | - | LEU2 | [35] |
p426ADH | - | URA3 | [35] |
YEpBIO-SBH1TM-M1 | BIO-SBH1(50-75, I65T F66S V73A I74T) | URA3 | This study |
YEpBIO-SBH1TM-M2 | BIO-SBH1(50-75, A70S) | URA3 | This study |
YEpBIO-SBH1TM-M3 | BIO-SBH1(50-75, F59L V62A F66L V69A L71S I74T) | URA3 | This study |
YEpBIO-SBH1TM-M4 | BIO-SBH1(50-75, L60P) | URA3 | This study |
YEpBIO-SBH1TM-M5 | BIO-SBH1(50-75, F64S S67P H72R) | URA3 | This study |
YEpBIO-SBH1TM-M6 | BIO-SBH1(50-75, I65V S67P) | URA3 | This study |
YEpBIO-SBH1TM-M7 | BIO-SBH1(50-75, V57A I65N L71P) | URA3 | This study |
YEpBIO-SBH1TM-M8 | BIO-SBH1(50-75, V57E L60P F66S) | URA3 | This study |