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Table 1 Receptor expression levels in HME cell lines

From: HER/ErbB receptor interactions and signaling patterns in human mammary epithelial cells

Clone Order of gene transfer Drug selection EGFR (103/cell) HER2 (103/cell) HER3 (103/cell)
HER2-/3- Parental none 200a ± 8 30b 2c, d
HER2+/3- HER2 into HER2-/3- G418 151b ± 5 600a ± 19 2c, d
HER2-/3+ HER3 into HER2-/3- Puromycin 200b ± 7 30b 28 ± 1c
HER2+/3+ HER3 into HER2+/3- G418 and Puromycin 84b ± 7 625b ± 34 28 ± 3c
  1. a An EGFR expression level of 200 K for the HER2-/3- cell line and a HER2 expression level of 600 K for HER2+/3- cell line were reported previously [42].
  2. b These EGFR and HER2 expression levels were calculated by normalizing the receptor masses measured in ELISA experiments with the respective EGFR and HER2 expression levels shown in item (a) above.
  3. c These HER3 expression levels were calculated by normalizing the receptor masses measured in ELISA experiments with the EGFR receptor expression level shown in item (a) above. Detected expression values were validated by re-computing them based on relative readings with respect to HER2 expression.
  4. d This is also roughly the detection limit in our ELISA experiments.