Figure 4From: RNAi mediated acute depletion of Retinoblastoma protein (pRb) promotes aneuploidy in human primary cells via micronuclei formationEvaluation of cell growth at different times after RB silencing. A) Decreased proliferation rates starting 48 h up to 7 days from transfection in RB/PLK1 and RB/AURORA-A co-depleted cells (double knock-down) in comparison with both untransfected and pRb-depleted cells. B) Western blot analysis showing increased Cyclin-E protein levels in pRb-depleted cells as well as in cells with knock-down of both RB and AURORA-A or PLK1, and increased Cyclin-A protein levels in RB/AURORA-A co-depleted cells. Cells with knock-down of both RB and AURORA-A or PLK1 but not with RB knock-down showed increased p21Cip1 levels. β-tubulin was used as a loading control of protein extracts.Back to article page