TY - JOUR AU - Akbari, Omar S. AU - Oliver, Daniel AU - Eyer, Katie AU - Pai, Chi-Yun PY - 2009 DA - 2009/01/29 TI - An Entry/Gateway® cloning system for general expression of genes with molecular tags in Drosophila melanogaster JO - BMC Cell Biology SP - 8 VL - 10 IS - 1 AB - Tagged fusion proteins are priceless tools for monitoring the activities of biomolecules in living cells. However, over-expression of fusion proteins sometimes leads to the unwanted lethality or developmental defects. Therefore, vectors that can express tagged proteins at physiological levels are desirable tools for studying dosage-sensitive proteins. We developed a set of Entry/Gateway® vectors for expressing fluorescent fusion proteins in Drosophila melanogaster. The vectors were used to generate fluorescent CP190 which is a component of the gypsy chromatin insulator. We used the fluorescent CP190 to study the dynamic movement of related chromatin insulators in living cells. SN - 1471-2121 UR - https://doi.org/10.1186/1471-2121-10-8 DO - 10.1186/1471-2121-10-8 ID - Akbari2009 ER -