Structure of the pGWS and P-destination vectors. (A-B) The detailed structure of P-DEST vectors with N-terminal tags (A), and with C-terminal tags (B). The sequences and the reading frame from the epitope tags are shown below the map. The attR1 and attR2 sequences for Clonase II® recombination are shaded. (C) The structure and sequences of the pGWS. The circular pGWS has a unique SmaI site which is a restriction enzyme that can cut pGWS into a linear DNA with two blunted ends. The 3'-protruding ends after Taq T-tailing for TA cloning are shown in brackets. The reading frame after LR recombination with P-DEST vectors is indicated.