Figure 3

The pGWS entry clones are efficient in the Clonase II LR reactions. (A) The diagram of LR reaction of the pGWS.eGFP entry clone and pDEST17. Shown are features of the two parental plasmids and the recombined product encoding the eGFP protein with poly-His tag. The arrows indicate the primers for determining the bacteria colonies that contain the correctly recombined plasmid. (B) Colonies of the eGFP-His expressing bacteria are fluorescent. (C) The bacterial lysate and the His-bind-chromatography-purified eGFP-His protein were analyzed by SDS-PAGE and stained with Coomassie blue. The arrow points to the purified His-eGFP protein.