Figure 3

Endogenous hnRNP L protein co-localises with Sam68 within SNBs. Representative images of LNCaP and Saos-2 cells captured by confocal laser scanning microscopy using indirect immunofluorescence and antibodies specific to Sam68, hnRNP L, hnRNP LL and SC35. (A and B) Sam68 co-localises with hnRNP L to SNBs (arrowed), but not with SC35 to splicing speckles (C). (D) hnRNP LL exhibits a different subnuclear localisation to hnRNP L, which is again distinct from SC35-containing splicing speckles (E), and does not co-localise to SNBs (arrowed). (Bar = 10 μm).