Figure 4

An ectopically-expressed hnRNP L fusion protein localises within SNBs. Representative indirect immunofluorescence images of HeLa cells transfected with vectors encoding hnRNP L-GST (A), hnRNP LL-GST (B), or GST alone (C), and captured by confocal laser scanning microscopy using indirect immunofluorescence and antibodies to Sam68 and GST. (A) Direct co-localisation within SNBs of endogenous Sam68 and ectopically-expressed hnRNP L-GST fusion protein (arrowed). (B) No co-localisation within SNBs was observed between endogenous Sam68 and the ectopically-expressed hnRNP LL-GST fusion protein. (C) GST protein was generally distributed within the nucleus and cytoplasm and showed no subnuclear localisation within SNBs, (Bar = 10 μm).