Peptide localization in EPH4 cells. EPH4 cells grown on glass slides were treated with FITC labeled LYHY (A) or LYQY (B) peptides (350 μM) for 6 hr (pseudocolored red). After fixation, cells were stained with antibodies against occludin (green pseudocolor) and E-cadherin (green pseudocolor) and with DAPI for nuclei (blue). LYHY accumulated in regions enriched in non-junctional occludin, denoted by arrowhead. LYQY did not alter occludin distribution although it appeared to localize in the cytoplasm of some cells. E-cadherin distribution was not altered by LYHY.