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Figure 4 | BMC Cell Biology

Figure 4

From: The Golgi α-1,6 mannosyltransferase KlOch1p of Kluyveromyces lactis is required for Ca2+/calmodulin-based signaling and for proper mitochondrial functionality

Figure 4

Functional mitochondrial analysis of Kloch1-1 cells transformed with CpKlCMD1 plasmid. (A) Estimation of ROS accumulation in WT cells (black bar) and Kloch1-1 mutant strain harbouring empty (dark grey bar) or CpKlCMD1 (light grey bar) vectors by DHR-staining after growth to exponential phase in SD medium. The values were the mean of three independent experiments and showed an SD < 10%. (B) DASPMI staining of mutant cells transformed with centromeric (left side of the panel) or multicopy (right side of the panel) plasmids containing KlCMD1 gene.

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