Figure 5
Altered calcium homeostasis in Kloch1-1 mutant. (A) Growth of indicated yeast strains in the presence of 20 mM EGTA. YPD-EGTA plates with and without 20 mM CaCl2 were spotted with 5 μl of 10-fold serial dilutions of cells from exponential cultures and growth at 28°C was monitored after 3 d; three independent transformants have been checked, obtaining identical results. (B) Intracellular calcium content in WT (black bar) and Kloch1-1 (dark grey bar) cells measured with FURA-2 AM, expressed as the ratio of fluorescence excitation intensities (340/380 nm). Ca2+ ion measurements of Klpmr1 Δ strain (light grey bar) was also reported as a control.