Figure 1
From: Analysis of replication factories in human cells by super-resolution light microscopy
RPA and PCNA labelled replication factories. A and B: MRC5 cells were pulse labelled for 10 minutes with 40 μ M EdU to label newly synthesised DNA which was then visualised in the green channel alongside indirect immunofluorescence (in magenta) from anti-PCNA or -RPA antibodies respectively. C and D: cells were co-labelled with anti-RPA monoclonal and anti-PCNA polyclonal antibodies in green and magenta (RPA-green in C and PCNA-green in D). Scale bars = 2 μ m.