CDK2 and cyclin E colocalize with FUS-DDIT3 in transiently transfected HT1080 cells. The endogenous distribution of CDK2 and cyclin E seen in red is detected by antiserum specific for these proteins. Ectopically expressed GFP-tagged FUS-DDIT3 is shown in green. The DAPI dye is used to stain nuclei blue. CDK4 was not seen to accumulate in FUS-DDIT3-containing granules and no granules were formed in cells overexpressing the GFP protein only. Bars indicate 5 μm.