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Table 2 Comparison of intracellular solutions

From: Intracellular diffusion restrictions in isolated cardiomyocytes from rainbow trout

Compound Mammalian R-solution KCl MiR05 Fish R-solution
CaK2EGTA 2.77    
K2EGTA 7.23    
Imidazole 20    
K-MES 100    
KCl   125   20
Sucrose    110  
K-lactobionate    60 85
MgCl2 1.38 3 3 3
K2HPO4/KH2PO4 3 5 10 5
Taurine 20   20 20
Dithiothreitol 0.3 0.3   0.3
EGTA   0.4 0.5 0.4
Hepes   20 20 20
Glutamate 5 5 5 5
Malate 2 2 2 2
BSA 2 mg/ml 2 mg/ml 1 mg/ml 2 mg/ml
pH 7.1 7.1 7.1 7.1
Ionic strength 142 142 95 122
Osmolarity 288 323 330 301
  1. Composition of the mammalian R-solution, KCl-solution (Oroboros, Austria), mitomed MiR05 (Oroboros, Austria) used for mammalian cardiomyocytes and the fish R-solution developed for the present study on permeabilized trout cardiomyocytes. All units are in mM except osmolarity, which is in mOsm.