Intracellular diffusion restrictions in isolated cardiomyocytes from rainbow trout

Table 2 Comparison of intracellular solutions

Compound	Mammalian R-solution	KCl	MiR05	Fish R-solution
CaK₂EGTA	2.77
K₂EGTA	7.23
Imidazole	20
K-MES	100
KCl		125		20
Sucrose			110
K-lactobionate			60	85
MgCl₂	1.38	3	3	3
K₂HPO₄/KH₂PO₄	3	5	10	5
Taurine	20		20	20
Dithiothreitol	0.3	0.3		0.3
EGTA		0.4	0.5	0.4
Hepes		20	20	20
Glutamate	5	5	5	5
Malate	2	2	2	2
BSA	2 mg/ml	2 mg/ml	1 mg/ml	2 mg/ml
pH	7.1	7.1	7.1	7.1
Ionic strength	142	142	95	122
Osmolarity	288	323	330	301

Composition of the mammalian R-solution, KCl-solution (Oroboros, Austria), mitomed MiR05 (Oroboros, Austria) used for mammalian cardiomyocytes and the fish R-solution developed for the present study on permeabilized trout cardiomyocytes. All units are in mM except osmolarity, which is in mOsm.

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