Figure 3From: Murine mesenchymal progenitor cells from different tissues differentiated via mesenchymal microspheres into the mesodermal directionAfter generation of mesenchymal microspheres (MMS) by hanging drop cultivation the standardized cellular aggregates are plated on plastic culture dishes (A); at 0 d specific medium is applied to induce differentiation and the MMS grow out. Alcian blue (AB) staining (bottom) of cryosectioned "micromass bodies" (MMB) derived from murine bone marrow (BM; B), perirenal adipose tissue (PAT; C), and mediastinal stromal tissue (MST; D) as well as AB staining of MMS outgrowths (E-G) demonstrates chondrogenic differentiation of the mesenchymal progenitor cells with a maximum at day 25 of differentiation. Bar = 100 μm.Back to article page