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Figure 7 | BMC Cell Biology

Figure 7

From: Complete reversal of epithelial to mesenchymal transition requires inhibition of both ZEB expression and the Rho pathway

Figure 7

ZEB1 and ZEB2 depletion by shRNAs in NMuMG cells attenuates F-actin stress fibers. Cells were treated as indicated with 100 pM TGF-β1 for 48 hours followed by an additional 72 hour incubation with a lentivirus encoding pLKO.1 (a control shRNA) or lentiviruses encoding shRNAs against ZEB1 and ZEB2, with 1 μM Y27632 or 10 μM SP600125 added during the last 24 hour incubation with the shRNAs. F-actin was visualized by phalloidin. Cells were viewed at a 400× magnification. White arrows point to stress fibers.

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