Mechano-transduction in periodontal ligament cells identifies activated states of MAP-kinases p42/44 and p38-stress kinase as a mechanism for MMP-13 expression

Table 2 Strain-induced pathway-specific quantitative mRNA-expression changes in PDL cells

gene symbol	relative gene expression value	gene symbol	relative gene expression value	gene symbol	relative gene expression value	gene symbol	relative gene expression value	gene symbol	relative gene expression value
*extracellular matrix and integrin pathway*		*growth factor pathway*		*growth factor pathway*		*growth factor pathway*		*MAP-Kinase signalling and cell cycle pathway*
COL11A1	2.08	BMP1	-2.07	FGF11	2.14	GDF8	10.56	CCNA1	2.38
COL14A1	-5.43	BMP2	-36.76	FGF14	6.06	GDNF	-5.28	CCNB1	2.07
COL1A1	-6.23	BMP4	5.66	FGF17	4.00	IGF1	-8.00	CDKN2A	3.73
COL4A2	-2.06	BMP5	3.73	FGF2	-17.15	IL11	-10.20	CDKN2C	2.14
HAS1	-2.53	BMP6	-4.29	FGF22	5.10	IL12B	2.38	EGR1	9.85
ITGA3	2.75	BMP7	2.46	FGF23	2.30	IL18	3.73	FOS	59.89
ITGA8	-2.06	CSF1	-4.44	FGF6	4.76	IL1A	-8.00	MAP2K6	8.28
MMP10	-6.68	CSF2	-22.63	FGF7	-14.93	IL1B	-42.22	MAP4K1	4.92
MMP12	6.32	CSF3	-17.75	FGF9	3.25	IL2	6.06	MAPK6	5.86
MMP13	2.51	CXCL1	-115.36	GDF10	2.30	SPP1	3.36	MAPK8IP2	10.58
		ECGF1	-12.13			TGFB1	-3.86
		EREG	-26.91			VEGFA	-5.86
						VEGFC	-2.38

Values of relative increase or decrease in gene expression represent the mean of two independent RT-Profiler experiments and were normalised using an experimental internal index of housekeeping genes. All expression values above 2 and below -2 were considered per definition as significantly modulated compared with unstrained basal control expression of the respective gene on each RT-Profiler.

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