Figure 1

Protein structure and expression levels of CP190 deletion mutants. (A) Schematic diagram of CP190 deletion mutants and their genetic complementation phenotypes. The full-length Cp190 is tagged with mRFP (mRFP-CP190). Each CP190 mutant contains a deletion of one of the functional domains. CP190dBTB lacks the BTB domain and is tagged with myc (myc-CP190dBTB) or with GFP (GFP-CP190dBTB, not shown); CP190dZnF lacks all three zinc fingers and is tagged with GFP (GFP-CP190dZnF); CP190ΔM lacks the centrosomal targeting domain (CENT); CP190BTB-D lacks CENT, zinc fingers and the E-rich domain and is tagged with GFP (GFP-CP190BTB-D); CP190BTB has only the BTB domain and is tagged with GFP (GFP-CP190BTB). The CP190dCT(En15) is the predicted protein from the EMS-induced CP190^En15mutant, which lacks two zinc fingers and the E-rich region. The amino acids at the junction of each deletion are indicated. (B-C) Expression of the mutated Cp190 proteins revealed by the anti-CP190 immunoblot (B) or by anti-GFP immunoblots (C). All transgenic lines were crossed into the homozygous CP190³background. Proteins extracted from about two 3^rd instar larvae containing the indicated transgene were loaded per each lane. Similar results were also obtained from pupae 24-48 hours after pupation. Underneath the blots were stripped filters re-probed with the anti-actin antibody as a loading control.