Figure 4

The C-terminal E-rich domain is essential to Cp190's insulator function. (A) Genetic assays to test functionality of the gypsy insulator in CP190^E15. Shown are morphological phenotypes of the y²w omb^P1-D11ct⁶; mod(mdg4)^T6e female (left, control) and the y²w omb^P1-D11ct⁶; CP190^En15mod(mdg4)^T6e/mod(mdg4)^T6e female (right, En15). The En15 fly has a darker abdomen cuticle color (enhanced y²phenotype) compared to the control fly. The arrowhead points to the partially suppressed ct⁶wing shape phenotype which lacks some wing margin bristle cells. The arrow points to the fully suppressed ct⁶wing shape (enhanced ct⁶phenotype). The omb^P1-D11pigmentation pattern of the eye of the control female fly is shown on the upper left and the omb^P1-D11pigmentation pattern of the En15 female which has expanded white region in the equatorial part of the eye (enhanced omb^P1-D11phenotype) is shown on the lower right. (B) The body cuticle pigmentation (upper panel) and the wing shape (lower panel) of the CP190^En15/CP190^P11 mutant (left column) and CP190⁺flies (right column). Arrows point to different pigmentation of the abdomens.