The C-terminal E-rich domain is essential to Cp190's insulator function. (A) Genetic assays to test functionality of the gypsy insulator in CP190E15. Shown are morphological phenotypes of the y2w ombP1-D11ct6; mod(mdg4)T6e female (left, control) and the y2w ombP1-D11ct6; CP190En15mod(mdg4)T6e/mod(mdg4)T6e female (right, En15). The En15 fly has a darker abdomen cuticle color (enhanced y2phenotype) compared to the control fly. The arrowhead points to the partially suppressed ct6wing shape phenotype which lacks some wing margin bristle cells. The arrow points to the fully suppressed ct6wing shape (enhanced ct6phenotype). The ombP1-D11pigmentation pattern of the eye of the control female fly is shown on the upper left and the ombP1-D11pigmentation pattern of the En15 female which has expanded white region in the equatorial part of the eye (enhanced ombP1-D11phenotype) is shown on the lower right. (B) The body cuticle pigmentation (upper panel) and the wing shape (lower panel) of the CP190En15/CP190P11 mutant (left column) and CP190+flies (right column). Arrows point to different pigmentation of the abdomens.