Figure 4

Expression of α8 integrin in vascular smooth muscle cells. Real-time RT-PCR analysis of α8 integrin expression in wild type (wt) vascular smooth muscle cells cultivated in passage 1 or 10 (A). Real-time RT-PCR analysis of α8 integrin expression in freshly isolated wt and α8-/- vascular smooth muscle cells (B). Western blot analysis of α8 integrin expression in freshly isolated wt and α8-/- vascular smooth muscle cells (C). Amido black (Abl) staining of the blot served as loading control. Results are representative for at least 3 similar experiments. Data are means ± sd. * p < 0.05 vs. freshly isolated cells or wt, respectively.