Figure 3

Immunocytochemical analysis of in vitro differentiated TrES1. TrES1 was differentiated toward neuronal lineage in vitro using a step-wise differentiation protocol. Antibodies specific for neural progenitor cells (nestin), glial fibrillary acidic protein (GFAP), and mutant htt (mEM48) were used for immunostaining at different differentiation stages: N2, N3-1 week (N3-1w), and N3-4 weeks (N3-4w). At N2 stage, all cells were stained with Nestin and some were stained positive with mEM48. At N3-1w and N3-4w, cells were stained with GFAP, βIII-tubulin and mEM48. First column-brightfield images; second column-epifluorescent images of GFP; third column-DNA staining with Hoechst; fourth column-immunostaining with specific antibodies, and fifth column-overlay images of the third and fourth columns. Insets are images of selected nuclei with nuclear inclusions at higher magnification.