Figure 4

Expression pattern of mutant htt in neural differentiated TrES1. (A) Expression levels of mutant htt at various developmental stages were determined by Q-PCR. YRES4 is a WT-monkey ES cell line and was used as a control. The expression levels of mutant htt in differentiated TrES1 were significantly increased at N2, N3-1w and N3-4w compared to undifferentiated TrES1 (ES) and YRES4 at all differentiation stages. Columns with the same letter indicate no significant difference (P > 0.05). (B) Western blot analysis using mEM48 revealed a gradual increase of oligomeric transgenic mutant htt as TrES1 progresses during neural differentiation (N3-4w > N3-1w > N2) whereas no high molecular weight mutant htt aggregates was detected in undifferentiated TrES1 or YRES4 at all stages. (C) Increase of cells expressing mutant htt detected by mEM48 was observed as differentiation progresses. Columns with the same letter indicate no significant difference (P > 0.05). (D) Expression pattern of mutant htt was categorized into four groups: soluble form, 1-to-5 nuclear inclusions (NIs), six to10 NIs, and more than 10 NIs. Columns of the same category with the same letter indicate no significant different (P > 0.05).