Figure 6
From: R-Ras regulates β1-integrin trafficking via effects on membrane ruffling and endocytosis
β 1 -integrin traffics through R-Ras-positive vesicles. Time course images of (A) a GFP-R-Ras(wt) transfected Cos7 cell incubated in Alexa Fluor555 (red) labeled β1-integrin antibody solution for 30 mins reveal the interplay of R-Ras and β1-integrin (See Additional file 8, Movie S5). (B, C) The inset of images from the boxed region in A acquired at one minute intervals show that R-Ras and β1-integrin traffic together to large endosomal vesicles. (D) Endocytosed components are then repackaged together and bud off from these organelles. Scale bar is 20 μm.