Figure 4

Clathrin-coated vesicle dynamics revealed by photoconverting expressed mEos2-clathrin light chain. Numerous clathrin-coated vesicles are visible in the cytoplasm of an mEos2-clathrin light chain expressing HeLa cell (panel 1, row 1). A distal portion of this vesicle pool was photoconverted within about 10 sec (circled). Within 2-5 minutes after photoconversion, photoconverted and remaining, unconverted vesicles moved laterally and intermixed (red, green and yellow vesicles, the resulting color when red and green fluorescence colocalize, arrows), consistent with the known dynamic mobility and structural composition of these vesicles. N = Cell nucleus.