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Figure 5 | BMC Cell Biology

Figure 5

From: Integrin-linked kinase functions as a downstream signal of platelet-derived growth factor to regulate actin polymerization and vascular smooth muscle cell migration

Figure 5

ILK regulates PDGF-induced SMC migration through a p38 MAPK-dependent pathway. A) Kinase deficient mutant of ILK significantly reduced PDGF-induced SMC migration in a Traswell Boyden Chamber migration assay. As shown, expression of a wild type form of ILK rescued SMC migratory response indicating the specificity of ILK inhibition by the adenoviral construct. B) Over-expression of an active form of MKK3/MKK6 (specific upstream activators of p38 MAPK), but not a wild type form of p38 MAPK, rescued SMC migration in response to PDGF-BB indicating that an active form of ILK is required for the initiation of a p38 MAPK-dependent migratory response to PDGF-BB in mouse aortic SMCs. (n = 3 cell cultures for each independent experiments).

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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