Figure 5
From: Role of casein kinase 1 in the glucose sensor-mediated signaling pathway in yeast
Direct interaction of Yck1/2 with the glucose sensors is not required for degradation of Mth1. (A) Subcellular localization of GFP-Yck1 and GFP-Yck2 expressed in wild type (BY4742) and akr1Δ mutant (JKY56) strains was examined by fluorescence microscopy. (B) Western blot analysis of GFP-Mth1 in wild-type and akr1Δ mutant strains. Yeast cells were grown as described in Figure 2.