Figure 1
Renewal and differentiation capacity of ADSCs from old, adult, and young mice. A. Doubling time of P0 ADSCs. *:P < 0.04, N = 5 for young ADSCs, N = 7 for old ADSCs. B. Number of small (5-10 cells/colony), medium (10-50 cells/colony), and large (> 50 cells/colony) colonies formed after 5 or 8 days of culture after initially plating 5000 young, adult, or old P0 ADSCs. Young ADSCs formed significantly more large colonies than either adult or old ADSCs. *1: P < 0.04; **2: P < 0.006 between adult and young ADSCs for the 5- and 8-day colonies; *3: P < 0.02; **4: P < 0.004 between old and young ADSCs for the 5- and 8-day colonies. ADSCs from the same mouse were plated in triplicates. N = 8. C. Osteogenic (von Kossa staining), myogenic (Liu's staining), and adipogenic (Oil-Red O staining) differentiation in young and old P1 ADSCs responding to differentiating medium after 21 days. The scale bars are 2.0 mm (von Kossa) and 500 μm (Liu's).