Figure 4
A. ARS staining for calcium deposition in osteogenically-induced young, adult, and old ADSCs with or without pre-exposure to mechanical strain. B. Left Osteogenic colonies from young and old ADSCs with or without pre-strain. Young ADSCs have stronger ARS staining than old cells. No difference in ARS staining caused by pre-strain was found. Scale bars = 500 μm. Right The expression of Runx2 and Bglap1 in young and old ADSCs with or without pre-strain. N = 8. *: P < 0.05, compared to non-strained young ADSCs.