Figure 5

Pre-strain does not enhance osteogenic differentiation in adult ADSCs. A. The extracted ARS stain normalized to the DNA content of adult ADSCs which were either pre-strained or unstrained, followed by osteogenic induction for 5, 14, or 21 days. A gradual increase in ARS stain was found following osteogenic induction, but no significant difference between the pre-strain and non-strain groups was found. N = 6. B. ARS staining of osteogenic colonies (day 14) of adult ADSCs with or without pre-strain. Scale bars = 2.0 mm. Ethidium bromide (EB) staining of the same well is shown below. White pixels in the culture well represent positive EB staining and the pre-strained ADSCs formed more colonies than non-strained ADSCs. C. Expression of Runx2 and Bglap1 in pre-strained or non-strained adult ADSCs after 14 days of osteogenesis. No significant difference was found between pre-strained and non-strained samples. N = 6.