Figure 4

Sus1 interacts with Dhh1 and co-localise with Pab1. (A) Sus1 physically interacts with Dhh1. Sus1 co-purification with Dhh1 was revealed by western-blot of Sus1-TAP calmoduline eluates purificied from wild-type (wt), and dhh1Δ cells using anti-Dhh1 antibodies (left panel). Prior to purification, Sus1 and Dhh1 presences were confirmed by western blot analysis of whole cell extracts (WCE) (right panel). Lower panel shows the enriched calmoduline eluates from Sus1-TAP (wt), and Sus1-TAPdhh1Δ purifications analysed by SDS 4-12% gradient polyacrylamide gel electrophoresis stained with Coomassie (B) MudPIT analysis of Sus1-TAP. List of proteins co-purified with Sus1 identified by MudPIT. PBs (P-bodies); SGs (stress granules); ND (Not Determined) (C) Sus1 is present at cytoplasmic Pab1-containing granules. Sus1 co-localises with Pab1 in stationary phase.