TY - JOUR AU - Bartfeld, Sina AU - Hess, Simone AU - Bauer, Bianca AU - Machuy, Nikolaus AU - Ogilvie, Lesley A. AU - Schuchhardt, Johannes AU - Meyer, Thomas F. PY - 2010 DA - 2010/03/16 TI - High-throughput and single-cell imaging of NF-κB oscillations using monoclonal cell lines JO - BMC Cell Biology SP - 21 VL - 11 IS - 1 AB - The nuclear factor-κB (NF-κB) family of transcription factors plays a role in a wide range of cellular processes including the immune response and cellular growth. In addition, deregulation of the NF-κB system has been associated with a number of disease states, including cancer. Therefore, insight into the regulation of NF-κB activation has crucial medical relevance, holding promise for novel drug target discovery. Transcription of NF-κB-induced genes is regulated by differential dynamics of single NF-κB subunits, but only a few methods are currently being applied to study dynamics. In particular, while oscillations of NF-κB activation have been observed in response to the cytokine tumor necrosis factor α (TNFα), little is known about the occurrence of oscillations in response to bacterial infections. SN - 1471-2121 UR - https://doi.org/10.1186/1471-2121-11-21 DO - 10.1186/1471-2121-11-21 ID - Bartfeld2010 ER -