Figure 3
From: High-throughput and single-cell imaging of NF-κB oscillations using monoclonal cell lines
Inducer-specific activation profiles of cell lines A549 SIB01, AGS SIB02 and L929 SIB01. The cell lines were activated with the indicated inducer for times between 0 and 6 h: A549 SIB01 was activated for 0-345 min, AGS SIB02 and L929 SIB01 for 0-270 min, each at intervals of 15 min. Cells were fixed, stained with Hoechst 33342 and then analyzed with automated microscopy and image analysis software. For each cell line, single cells were analyzed and the mean percentage of cells with nuclear p65-GFP per well was calculated as described in Figure 2. Mean percentages from duplicate experiments are shown as bars. Results are representative of at least three independent experiments. Standard deviations are not shown for graphical reasons. One data point is missing in AGS H. pylori MOI 10 due to technical issues.