Figure 9

Electron microscopy and quantification of TIL of human MSCs. Independently on exposition to magnetic fields, complexes of SPIO (black arrows) were localized on the surface of the MSCs without detectable uptake of SPIO into the cells directly after labeling (A = JPR-MSC + m (0 h), B = JPR-MSC + m (24 h), C = JPR-MSC - m). Seven days after labeling, exposition to magnetic fields led to a significant (*) enhancement of the TIL of MSCs (JPR-MSC + m(0 h), JPR-MSC + m(24 h)) compared to labeled MSCs without exposition to magnetic fields (JPR-MSC - m). Immediate exposition to magnetic fields (JPR-MSC + m(0 h)) was more effective than exposition after 24 h (JPR-MSC + m(24 h)) (D). Eleven days after labeling, the highest TIL was again achieved after immediate exposition to magnetic fields (JPR-MSC + m(0 h)), whereas the TIL of the labeled MSCs exposed after 24 h to magnetic fields (JPR-MSC + m(24 h)) was not different from the TIL of the labeled MSCs without exposition to magnetic fields (JPR-MSC - m) (E). Error bars: SEM.