Figure 2
Delay in repair of UV-induced DNA damage induced by exogenously expressed CC3. (A). U373 clonal populations, control (U3neo) and stably expressing CC3 (U3cc3), were irradiated with 15 J/m2 UVC and harvested at different times after exposure. After lysis and denaturation, cells were subjected to Comet assay analysis according to the manufacturer's (Trevigen) protocol. (B). Cells were exposed to UV as in A, and the cellular DNA was isolated at the times indicated. The repair of CPD lesions was quantified using ELISA with an anti-CPD antibody. All results are average of three experiments. (C). Proliferation of U373 clones subjected to 15 J/m2 UVC in a short-term assay. Cells were analyzed as described in Materials and methods; results are expressed as percent of untreated cultures; experiments were performed three times with cells plated in triplicate or quadruplicate for each experiment.