Figure 3
Mild impairment of the repair of UV induced DNA lesions by abrogation of expression of endogenous CC3. (A). Expression of CC3 in MCF10A cells infected with a control lentivirus and a CC3 siRNA expressing virus. (B). MCF10A cells were subjected to UVC irradiation at 10 J/m2, and harvested at different times after exposure. Relative repair of CPD lesions was quantified using ELISA with an anti-CPD antibody. Data represent average of three independent experiments. (C). A representative Southwestern blot analysis of DNA from UV-exposed MCF7 cells with anti-CPD antibody. (D) CPD repair in MCF7 cells, control and with silenced CC3. All charts show the average and standard deviation of three individual experiments.