Figure 2

Investigation of the multipotent capacity of hADSCs using immunostaining and RT-PCR analysis. a, Cells were cultured for one week in selection media specifically designed for adipogenic differentiation. Adipogenic differentiation is shown with oil-red O staining and RT-PCR analysis for lipoprotein lipase. b, Cells were cultured for two weeks in selection media specifically designed for osteogenic differentiation. Osteogenic differentiation is shown with alkaline phosphatase staining and RT-PCR analysis for osteopontin and PTH receptor. c, Cells were cultured for three weeks in selection media specifically designed for chondrogenic differentiation. Chondrogenic differentiation is shown with Alcian blue staining and RT-PCR analysis for periecan and collagen type II. Scale bars measure 50 μm. GAPDH was used as a control. The intensity of each gene was normalized to GAPDH and these results were repeated at least five times. * P < 0.05, ** P < 0.01 compare with the primary hADSCs.