Figure 3From: Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point1Cells in the low Mcm6 cluster express loaded Mcm6. BJ1 cells were harvested at 0 and 24 hours after serum starvation and prepared for electrophoresis after extracting the cells with Triton X-100 and washing. Equal cell numbers were loaded. The immunoblots were developed on X-Ray film that were then imaged and quantified. At 24 h, Mcm6 intensity was reduced by 25% and PCNA intensity by 72%. Under the conditions for serum starving BJ1 cells, there was an average of 5% of cells in S, G2 and M.Back to article page