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Table 1 Fraction of remaining immuno-reactivity

From: Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point1

Antigen Immunoblota Cytometryb
Mcm6 0.6 ± 0.1 (3) 0.6
Cdc6 0.4 ± 0.0 (2) 0.3
PCNA 0.4 ± 0.1 (2) 0.2
  1. a Optical densities of bands from western blots of lysates from cells that were extracted with Triton X-100 prior to lysis were divided by the optical densities of bands from lysates. Means and standard deviations for multiple experiments (N) are listed.
  2. b Mean fluorescence intensities of Molt4 cells extracted with 0.5% Triton X-100 then fixed with methanol prior to staining were divided by the mean fluorescence intensities of Molt4 cells fixed in methanol then stained.