Figure 2

HAb18G/CD147 promotes HEK293ar cells survival by mediating cell-cell contacts. A. Western blotting to reveal HAb18G/CD147 expression in HEK293ar and parental HEK293 cells in suspension culture. Two major forms of HAb18G/CD147 (43-66 and 35 kDa) were analyzed. α-Tubulin was used as a loading control (n = 4-6). B. Immunofluorescence of HAb18G/CD147 under laser scanning confocal microscope (Olympus FV1000, Tokyo, Japan; n = 4-10). The nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole). Magnification: ×1200. C. Western blotting to reveal HAb18G/CD147 expression in HEK293ar cells in HAb18G/CD147 RNA interference (n = 4-6). α-Tubulin was used as a loading control. D. Comparison of the gray scale ratio of HAb18G/CD147/α-tubulin in HAb18G/CD147 RNA interference (n = 4-6). ** p < 0.01, siRNA versus scrambled. E. Flow cytometric quantification of anoikis after siRNA treatment (n = 4-6). Anoikis was determined as described in Fig. 1A. F. C ell-cell contacts formation with time after treatment of targeted HAb18G/CD147-siRNA under the phase-contrast microscope (n = 4-6). Magnification: × 400. Each value represents the mean ± SD of at least triplicate determinations. Results are the representative of three similar experiments.