Figure 4

HAb18G/CD147 knockdown reduces the expression of E-cadherin. A. E-cadherin expression and the formation of cell-cell contacts with time after HAb18G/CD147 siRNA treatment (n = 4-6). Magnification: × 400. B. Western blotting to reveal E-cadherin in HAb18G/CD147-siRNA-treated HEK293ar cells in suspension culture (n = 4-6). α-Tubulin was used as a loading control. C. Comparison of the gray scale ratio of E-cadherin/α-tubulin in HAb18G/CD147-siRNA treatment (n = 4-6). ** p < 0.01, siRNA versus scrambled. Each value represents the mean ± SD of triplicate determinations. Results are the representative of three similar experiments.