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Table 1 Expression levels of natural and recombinant bomapin in cells

From: Bomapin is a redox-sensitive nuclear serpin that affects responsiveness of myeloid progenitor cells to growth environment

Cell line Bomapin (ng bomapin/mg total protein)
HL-60 0.55 ± 0.02
U937 1.85 ± 0.29
THP-1 2.44 ± 0.58
K562 expressing bomapin-EGFP 1.29 ± 0.26
K562 expressing C395S bomapin-EGFP 1.23 ± 0.21
HT1080 expressing bomapin-EGFP 2.58 ± 0.32
  1. Exponentially growing cells were lysed in a lysis buffer containing protease inhibitor cocktail, and bomapin level was quantified by bomapin-specific ELISA (as described in Methods section).