Figure 1

Subcellular localization of Glycosyltransferase 25 domain 1 protein. Huh7 cells were transfected with full length Glycosyltransferase 25 domain 1 containing an internal Myc-tag (GLT25D1) (See for more details Methods). After 24 h of expression, cells were fixed and subjected to immunofluorescence analysis. Cells were double labeled for GLT25D1 by anti-Myc (shown in red) and for the cellular markers (shown in green). ER is visualized by anti-protein disulfide isomerase (PDI) (top panels), ER-Golgi intermediate compartment by anti-ERGIC53 (middle panels) or Golgi by anti-Giantin (bottom panels). Yellow observed in the overlay indicates overlap of red and green signal. Pearsons correlation of PDI and GLT25D1 is 0.58, ERGIC53 and GLT25D1 is 0.13, and Giantin and GLT25D1 is 0.04.