Figure 5

Co-localization of Glycosyl transferase 25 domain 1 with Mannose binding lectin and Lysyl hydroxylase 3. A) Huh7 cells were co-transfected with Mannose binding lectin fused to GFP (MBL-GFP) and full length GLT25D1 tagged with Myc internally (GLT25D1), and analyzed by immunofluorescence after 24 h of expression. MBL was detected directly due to GFP and is shown in green. GLT25D1 and lysyl hydroxylase 3 (LH3) were visualized using antibodies against Myc or LH3 and are presented in red and blue, respectively. When red and green signals overlap this is observed as yellow, red and blue as purple and red, blue and green as white. Pearson correlation of GLT25D1 and MBL is 0.53, GLT25D1 and LH3 is 0.42, and LH3 and MBL is 0.45. B) A profile plot with signal intensities from MBL (green), GLT25D1 (red) and LH3 (blue) along the line drawn in the overlay on the left.