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Figure 6 | BMC Cell Biology

Figure 6

From: Connective Tissue Growth Factor (CTGF/CCN2) enhances lactogenic differentiation of mammary epithelial cells via integrin-mediated cell adhesion

Figure 6

CTGF/CCN2 enhances adhesion of HC11 cells through the α6β1 integrin complex. HC11-TRE cells were resuspended in serum-free growth media + EGF (10 ng/ml) and seeded on microtiter wells coated with recombinant CTGF/CCN2 (2 μg/ml) or 1% BSA wells as described in Methods. Adherent cells were fixed , stained, and quantified by reading the absorbance at 570 nm. Data are means of quadruplicate samples, error bars represent standard error. *, , ■, p < 0.005. (A) HC11-TRE cells adhesion in serum-free growth media + EGF (10 ng/ml) with EDTA (2.5 mM), EDTA + Mg2+ (5 mM) or no addition. (B) HC11-TRE cells adhesion in serum-free growth media + EGF (10 ng/ml) with function-blocking antibodies or isotype controls (25 μg/ml).

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