CTGF/CCN2 contributes to the formation of focal adhesion complexes in HC11 cells. (A) HC11-TRE and HC11-TRE-CTGF cell protein lysates were used to detect levels of the indicated focal adhesion-related proteins by western blot. (B) HC11-TRE and HC11-TRE-CTGF cells were seeded in 0.5% serum-containing media with EGF (10 ng/ml) for 4 days. The cells were then fixed in paraformaldehyde and stained for the presence of vinculin with Alexaflor488-conjugated secondary antibody and a DAPI nuclear counterstain. In the vinculin panel focal adhesions are shown as punctate bodies primarily near the edges of cells. Magnification = 40×.