Figure 3
Angiotensin II induces PlGF mRNA (A) and protein levels (B) via AT 1 in EA.Hy 926 endothelial cells and HUASMCs. Cells were pretreated for 30 min with 10-6 mol/L Losartan (AT1R antagonist) or -6 mol/L PD123319 (AT2R antagonist) and then stimulated with 10-6 mol/L Ang II for 24 hours. (A) Q-PCR results: PlGF mRNA expression was assayed by Q-PCR. Experiments were performed five times with the similar results (n = 5 in each group). * indicates P < 0.05 vs control EA.Hy 926 cells or HUASMCs. # indicates P < 0.05 vs cells treated with Ang II. (B) PlGF protein production in the cell culture media was measured by ELISA (n = 3 in each group). * indicates P < 0.05 vs control EA.Hy 926 cells or HUASMCs. # indicates P < 0.05 vs cells treated with Ang II.