Figure 1

Myotube formation and muscle specific gene expression. A) Representative phase contrast images of C2C12 cells 3hrs post plating (subconfluent; SC), 24 h post plating (Day 0), 24 h after achieving confluence (Day 1), or 72 hrs following differentiation (Day 3). B) Myosin heavy chain protein content at subconfluent (SC), 24 h post plating (Day 0), 24 h after achieving confluence (Day 1), or 72 hrs following differentiation (Day 3). C) Representative images of MyoD and myogenin immunohistochemistry and the quantification of positive nuclei. * indicates significantly different from subconfluent (SC) values.